ca akt plasmid Search Results


90
Addgene inc active akt1 cdna
Active Akt1 Cdna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ca+akt+plasmid/HA-Akt+CA+(myr-HA-Akt)+(Plasmid+%2316244)/pmc07659178-76-9-17
Average 90 stars, based on 1 article reviews
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90
Shanghai GenePharma constitutively active akt (akt-ca) plasmids
Constitutively Active Akt (Akt Ca) Plasmids, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ca+akt+plasmid/constitutively+active+akt++akt+ca++plasmids/pmc08185508-43-66-78
Average 90 stars, based on 1 article reviews
constitutively active akt (akt-ca) plasmids - by Bioz Stars, 2026-10
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90
Hormel Health Labs mouse ca-akt1 in puseamp plasmid
Mouse Ca Akt1 In Puseamp Plasmid, supplied by Hormel Health Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ca+akt+plasmid/mouse+ca+akt1+in+puseamp+plasmid/pm15845651-85-32-41
Average 90 stars, based on 1 article reviews
mouse ca-akt1 in puseamp plasmid - by Bioz Stars, 2026-10
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90
SunBio Inc constitutively active akt (ca-akt) plasmid
Constitutively Active Akt (Ca Akt) Plasmid, supplied by SunBio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ca+akt+plasmid/constitutively+active+akt++ca+akt++plasmid/pm27631978-106-5-21
Average 90 stars, based on 1 article reviews
constitutively active akt (ca-akt) plasmid - by Bioz Stars, 2026-10
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90
Omega Bio Tek ca-akt plasmids
<t>Constitutively</t> <t>active</t> <t>AKT</t> <t>(CA-AKT)</t> significantly reverses the inhibitory effect of ABZ treatment on the migration and invasion of melanoma cells. (a-b) The CA-AKT plasmids were transiently transfected into A375 and B16-F10 cells, while the control group was transfected with the pcDNA3.1 (+) vector only. At 24 h after treatment with 0.4 μ M ABZ, western blot analysis was used to detect the expression levels of N-cadherin, E-cadherin, pAKT, pGSK-3 β (Ser9/Tyr216), and Snail in the cytoplasm of A375 and B16-F10 cells. The histograms (bottom) show the relative density of the detected proteins. (c-d) The wound healing area and relative wound closure rate (%) in melanoma cells were quantified and analyzed 24 h after treatment with 0.4 μ M ABZ. The histograms (bottom) show the relative wound closure rate for each group. (e-f) Results of the transwell invasion experiments for A375 and B16-F10 cells under different conditions. The histograms (bottom) show the number of invasive cells in each group. The data are expressed as means ± SD. All experiments were performed thrice. ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.
Ca Akt Plasmids, supplied by Omega Bio Tek, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ca+akt+plasmid/ca+akt+plasmids/pmc08712124-35-0-13
Average 90 stars, based on 1 article reviews
ca-akt plasmids - by Bioz Stars, 2026-10
90/100 stars
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Image Search Results


Constitutively active AKT (CA-AKT) significantly reverses the inhibitory effect of ABZ treatment on the migration and invasion of melanoma cells. (a-b) The CA-AKT plasmids were transiently transfected into A375 and B16-F10 cells, while the control group was transfected with the pcDNA3.1 (+) vector only. At 24 h after treatment with 0.4 μ M ABZ, western blot analysis was used to detect the expression levels of N-cadherin, E-cadherin, pAKT, pGSK-3 β (Ser9/Tyr216), and Snail in the cytoplasm of A375 and B16-F10 cells. The histograms (bottom) show the relative density of the detected proteins. (c-d) The wound healing area and relative wound closure rate (%) in melanoma cells were quantified and analyzed 24 h after treatment with 0.4 μ M ABZ. The histograms (bottom) show the relative wound closure rate for each group. (e-f) Results of the transwell invasion experiments for A375 and B16-F10 cells under different conditions. The histograms (bottom) show the number of invasive cells in each group. The data are expressed as means ± SD. All experiments were performed thrice. ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.

Journal: Journal of Oncology

Article Title: Low-Dose Albendazole Inhibits Epithelial-Mesenchymal Transition of Melanoma Cells by Enhancing Phosphorylated GSK-3 β /Tyr216 Accumulation

doi: 10.1155/2021/4475192

Figure Lengend Snippet: Constitutively active AKT (CA-AKT) significantly reverses the inhibitory effect of ABZ treatment on the migration and invasion of melanoma cells. (a-b) The CA-AKT plasmids were transiently transfected into A375 and B16-F10 cells, while the control group was transfected with the pcDNA3.1 (+) vector only. At 24 h after treatment with 0.4 μ M ABZ, western blot analysis was used to detect the expression levels of N-cadherin, E-cadherin, pAKT, pGSK-3 β (Ser9/Tyr216), and Snail in the cytoplasm of A375 and B16-F10 cells. The histograms (bottom) show the relative density of the detected proteins. (c-d) The wound healing area and relative wound closure rate (%) in melanoma cells were quantified and analyzed 24 h after treatment with 0.4 μ M ABZ. The histograms (bottom) show the relative wound closure rate for each group. (e-f) Results of the transwell invasion experiments for A375 and B16-F10 cells under different conditions. The histograms (bottom) show the number of invasive cells in each group. The data are expressed as means ± SD. All experiments were performed thrice. ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.

Article Snippet: Endotoxin-free CA-AKT plasmids (used for transfection) were extracted using Endo-Free Plasmid Midi Kit-fast (Omega Bio-tek, Norcross, GA, USA).

Techniques: Migration, Transfection, Plasmid Preparation, Western Blot, Expressing