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Image Search Results
Journal: Journal of Oncology
Article Title: Low-Dose Albendazole Inhibits Epithelial-Mesenchymal Transition of Melanoma Cells by Enhancing Phosphorylated GSK-3 β /Tyr216 Accumulation
doi: 10.1155/2021/4475192
Figure Lengend Snippet: Constitutively active AKT (CA-AKT) significantly reverses the inhibitory effect of ABZ treatment on the migration and invasion of melanoma cells. (a-b) The CA-AKT plasmids were transiently transfected into A375 and B16-F10 cells, while the control group was transfected with the pcDNA3.1 (+) vector only. At 24 h after treatment with 0.4 μ M ABZ, western blot analysis was used to detect the expression levels of N-cadherin, E-cadherin, pAKT, pGSK-3 β (Ser9/Tyr216), and Snail in the cytoplasm of A375 and B16-F10 cells. The histograms (bottom) show the relative density of the detected proteins. (c-d) The wound healing area and relative wound closure rate (%) in melanoma cells were quantified and analyzed 24 h after treatment with 0.4 μ M ABZ. The histograms (bottom) show the relative wound closure rate for each group. (e-f) Results of the transwell invasion experiments for A375 and B16-F10 cells under different conditions. The histograms (bottom) show the number of invasive cells in each group. The data are expressed as means ± SD. All experiments were performed thrice. ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.
Article Snippet:
Techniques: Migration, Transfection, Plasmid Preparation, Western Blot, Expressing